Description
This book provides an overview of scanning electron microscopy and its various applications in cell and molecular biology. It is divided into fourteen chapters written by experts in the field, and it is recommended for both beginners and advanced users.
Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM. Review: 'This book provides not only basic information that will be useful to those new to microscopy, but also tips from experienced users that will surely aid other experienced microscopists ... Recommended.' L. M. Baird, Choice '[This book] is interesting for both beginners and advanced users. It is divided into fourteen chapters written by thirty-six contributors from nine different countries, which underlines its broad scope.' Jean-Marie Volland, Marine Ecology
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