Description
This book is about neural cell culture and how to create and maintain these cultures. It is divided into chapters that cover different parts of the nervous system, and each chapter includes a dissection guide and step-by-step protocols. The book is aimed at researchers who want to study neural processes in vitro.
The immense complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study many types of neural processes at a cellular and molecular level. In recent years, the establishment of cell culture model systems for almost every region and cell type in the nervous system has been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms. Neural Cell Culture brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avian species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints and tips for success, advice, and background information.
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